The 12-string acoustic guitar, which was thought to be lost, was found in the home of a British couple after more than 50 years of not being seen or played.
It will now be put up for auction, with an estimated price range of £485,000 to £647,000.
According to auctioneers, it might establish a “new world record for the highest-selling Beatles guitar.”.
The Liverpool band performed You Have Got To Hide Your Love Away in the 1965 film Help! using the Hootenanny model, which was made by the German company Framus.
It was also utilized in the recording sessions for the songs It’s Only Love, I’ve Just Seen a Face, and Girl, and George Harrison’s rhythm track for Norwegian Wood.