Murray, who intends to retire later this year, underwent back surgery ten days ago.
The 37-year-old, who took home the titles at Wimbledon in 2013 and 2016, was scheduled to take on Czech player Tomas Machac on Tuesday at Centre Court.
“Unfortunately, despite working incredibly hard on his recovery since his operation just over a week ago, Andy has taken the very difficult decision not to play the singles this year,” the team stated in a statement.
He is obviously quite sad, but he has stated that he would participate in the doubles alongside.
On Saturday, former world number one Murray underwent surgery to remove a cyst near his spinal cord that was causing neurological pain in his right leg.
Having previously won Olympic gold in London 2012, Murray has been giving himself as much time as possible to get prepared for what will be an emotional farewell at the location of some of his greatest victories.
The back problem that flared up earlier this summer has complicated the Scot’s ambitions. It prompted him to withdraw from his June 19 Queen’s match against Australia’s Jordan Thompson after just five games.
However, he has battled to stay in shape because he intends to have some “closure” at the All England Club prior to quitting his professional career.